Dissociation Reagents
STEMCELL Technologies Dispase (1 U/mL), Size: 100 mL
Dispase is a protease that is suitable for the gentle dissociation of a wide variety of tissues. Incubation of minced tissue with pre-warmed dispase and gentle agitation will liberate cells with minimal cell damage. Pre-warmed dispase can also be used to harvest cells from tissue culture plastic. Unlike trypsin, dispase is not inhibited by serum. Dispase activity is inhibited by EDTA and EGTA. Dispase should be removed from cell suspensions by centrifuging the cells followed by washing the cells with buffer or culture medium.
STEMCELL Technologies STEMdiff™ Neural Rosette Selection Reagent, Size: 100 mL
STEMdiff™ Neural Rosette Selection Reagent is an enzyme-free reagent for the selective detachment of neural rosette clusters from adherent neural aggregates previously generated from human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium, without manual scraping. Collecting and re-plating rosette clusters after incubation with STEMdiff™ Neural Rosette Selection Reagent will yield highly pure populations of neural progenitor cells, which can be further sub-cultured as single cells.
Amsbio LLC Detachin Cell Detachment Solution
Detachin™ Cell Detachment Solution is a superior alternative to Trypsin/EDTA for gently detaching adherent cells from in vitro growth vessels. Detachin™ provides quick, gentle, and effective detachment of a wide variety of adherent cells, including primary cells, from all known tissue culture plastic ware. It contains protease and collagenase activities in an isotonic, phosphate buffer solution with EDTA.
Bioss Canine Parvovirus
Canine Parvovirus Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Bioss Parvovirus
Parvovirus Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Bioss Canine Parvovirus
Canine Parvovirus Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Bioss Canine Parvovirus
Canine Parvovirus Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
ILEX LIFE SCIENCES LLC TRYPSIN/EDTA P10-038100 100ML
PAN-Biotech Trypsin 0.05% / EDTA 0.02% in HBSS, w/o: Ca and Mg, w: Phenol Red (100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.05% / EDTA 0.02% in HBSS, w/o: Ca and Mg, w: Phenol Red is a porcine pancreas-derived enzyme plus EDTA in HBSS (Hanks' Balanced Salt Solution). Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate varies with cell type and culture age. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: With Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.05% Trypsin. EDTA: 0.02% EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC TRYPSIN/EDTA P10-029500 500ML
PAN-Biotech Trypsin 0.25% / 1 mM EDTA in HBSS, w/o: Ca and Mg, w: Phenol Red (500 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% / 1 mM EDTA in HBSS, w/o: Ca and Mg, w: Phenol Red is a porcine pancreas-derived enzyme plus EDTA in HBSS (Hanks' Balanced Salt Solution). Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate varies with cell type and culture age. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: With Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: 0.042 % (1.00 mM) EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC TRYPSIN/EDTA P10-036100 100ML
PAN-Biotech Trypsin 0.25% / 0.53 mM EDTA in HBSS, w/o: Ca and Mg, w: Phenol Red (100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% / 0.53 mM EDTA in HBSS, w/o: Ca and Mg, w: Phenol Red is a porcine pancreas-derived enzyme plus EDTA in HBSS (Hanks' Balanced Salt Solution). Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate varies with cell type and culture age. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: With Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: 0.53 mM EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC TRYPSINEDTA P10-023100 6X100ML
PAN-Biotech Trypsin 0.05% / EDTA 0.02% in DPBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.05% / EDTA 0.02% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.05% Trypsin. EDTA: 0.02% EDTA. STERILE: Yes. STORAGE TEMP.: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC TRYPSINEDTA P10-038100 6X100ML
PAN-Biotech Trypsin 0.05% / EDTA 0.02% in HBSS, w/o: Ca and Mg, w: Phenol Red (6 x 100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.05% / EDTA 0.02% in HBSS, w/o: Ca and Mg, w: Phenol Red is a porcine pancreas-derived enzyme plus EDTA in HBSS (Hanks' Balanced Salt Solution). Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate varies with cell type and culture age. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: With Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.05% Trypsin. EDTA: 0.02% EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC TRYPSIN/EDTA P10-027100 100ML
PAN-Biotech Trypsin 0.05% / EDTA 0.1% in DPBS, w/o: Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.05% / EDTA 0.1% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.05% Trypsin. EDTA: 0.1% EDTA. STERILE: Yes. STORAGE TEMP.: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).